| 13:30-14:00 |
Registration |
Leigh |
Anzio road foyer |
|
| 14:00-17:00 |
Introduction to the Linux command line / intro to R |
Gerrit / Katie |
Health - Wolfson Lab 1 |
Unix intro, Shell tutorial, R tutorial |
| Tuesday 24 October |
|
|
|
|
| 09:00-09:15 |
Welcome |
Nicky |
Health - Wolfson Lab 1 |
|
| 09:15-10:00 |
The human microbiome – overview of bacteria, fungi, and viruses |
Shantelle |
Health - Wolfson Lab 1 |
I, video 1 |
| 10:00-10:30 |
The correct study design to answer the research question at hand (16S vs whole genome) |
Shantelle |
Health - Wolfson Lab 1 |
II, video 1, video 2 |
| 10:30-11:00 |
Coffee / tea break |
|
IDM cafeteria |
|
| 11:00-12:00 |
Microbiome research: where are we now? |
Shantelle |
Health - Wolfson Lab 1 |
III, video 1, video 2, video 3 |
| 12:00-13:00 |
How important is sample collection and DNA/RNA extraction when profiling microbial communities |
Shantelle |
Health - Wolfson Lab 1 |
IV |
| 13:00-14:00 |
Lunch |
|
MAC club |
|
| 14:00-15:00 |
Library preparation for 16S rRNA sequencing |
Shantelle |
Health - Wolfson Lab 1 |
V |
| 15:00-15:30 |
Coffee / tea break |
|
IDM cafeteria |
|
| 15:30-17:00 |
16S rRNA sequencing bio-informatics pipeline: the theory |
Samson |
Health - Wolfson Lab 1 |
The microbiome: analysis of NGS data |
| Wednesday 25 October |
|
|
|
|
| 09:00-09:45 |
16S analysis pipeline - QC, OTU picking, classification, alignment |
Gerrit |
Health - Wolfson Lab 1 |
Data analysis of 16S rRNA gene amplicons |
| 09:45-10:30 |
Tutorial in usearch and QIIME |
Gerrit / Samson |
Health - Wolfson Lab 1 |
usearch and QIIME tutorial |
| 10:30-11:00 |
Coffee / tea break |
|
IDM cafeteria |
|
| 11:00-12:30 |
Tutorial in usearch and QIIME |
Gerrit / Samson |
Health - Wolfson Lab 1 |
|
| 12:30-13:30 |
Lunch |
|
MAC club |
|
| 13:30-15:00 |
Tutorial in usearch and QIIME |
Gerrit / Samson |
Health - Wolfson Lab 1 |
|
| 15:00-15:30 |
Coffee / tea break |
|
IDM cafeteria |
|
| 15:30-17:00 |
Tutorial in usearch and QIIME |
Gerrit / Samson |
Health - Wolfson Lab 1 |
|
| Thursday 26 October |
|
|
|
|
| 09:00-09:45 |
Downstream analysis in R - Phyloseq, NMF |
Katie / Jerome |
Health - Wolfson Lab 1 |
Downstream analysis intro, Downstream analysis in R tutorial |
| 09:45-10:30 |
Downstream analysis in R - Phyloseq, NMF |
Katie / Jerome |
Health - Wolfson Lab 1 |
|
| 10:30-11:00 |
Coffee / tea break |
|
IDM cafeteria |
|
| 11:00-12:30 |
Downstream analysis in R - Phyloseq, NMF |
Katie / Jerome |
Health - Wolfson Lab 1 |
|
| 12:30-13:30 |
Lunch |
|
MAC club |
|
| 13:30-15:00 |
Downstream analysis in R - Phyloseq, NMF |
Katie / Jerome |
Health - Wolfson Lab 1 |
|
| 15:00-15:30 |
Coffee / tea break |
|
IDM cafeteria |
|
| 15:30-17:00 |
Downstream analysis in R - Phyloseq, NMF |
Katie / Jerome |
Health - Wolfson Lab 1 |
|
| Friday 27 October |
|
|
|
|
| 09:00-10:00 |
Overview of shotgun sequence analysis |
Katie |
Aadil Moerat Conference Room |
Introduction to metagenomics |
| 10:00-10:30 |
Talk by CBIO researcher on their microbiome work |
Kirsty |
Aadil Moerat Conference Room |
Kirsty’s talk, Kirsty’s poster |
| 10:30-11:00 |
Coffee / tea break |
|
IDM cafeteria |
|
| 11:00-12:30 |
Working through parts not completed during practicals or helping participants with their own data. |
Gerrit / Katie / Samson / Jerome |
Health - Wolfson Lab 1 |
|
| 12:30-13:30 |
Lunch |
|
MAC club |
|
| 13:30-15:00 |
Working through parts not completed during practicals or helping participants with their own data. |
Gerrit / Katie / Samson / Jerome |
Health - Wolfson Lab 1 |
|
| 15:00-15:30 |
Coffee / tea break |
|
IDM cafeteria |
|
| 15:30-17:00 |
Working through parts not completed during practicals or helping participants with their own data. |
Gerrit / Katie / Samson / Jerome |
Health - Wolfson Lab 1 |
|